Production of fluorescent and cytotoxic K28 killer toxin variants through high cell density fermentation of recombinant Pichia pastoris

Table 3 PCR primers used in this study

Primer	Sequence (5′–3′)
3′ mCherry	agatctgtcgacgcggccgcTTACTTGTACAGCTCGTCCATGCCG
3′ mCherry^HDELR	agatctgtcgacgcggccgcTTAGCGTAGCTCATCGTGCTTGTACAGCTCGTCCATGCCGC
3′ mTFP^HDELR	agatctgtcgacgcggccgcTTAGCGTAGCTCATCGTGCTTGTACAGCTCGTCCATGCCGTC
3′ SOE β-mCherry	CCTCCTCGCCCTTGCTCACCATGCACCTTGCCTCGTCGTCACC
3′ SOE β-mTFP	GTCTCCTCGCCCTTCGTCACCATGCACCTTGCCTCGTCGTCACC
5′ K28 wo SP	agatctctcgagAAAAGAATGCCGACATCTGAGAGACAGCAGGG
5′ mCherry	agatctctcgcgAAAAGAATGCCGACATCTGAGAGACAGCAGGG
5′ mTFP	agatctctcgagAAAAGAATGGTGAGCAAGGGCGAGGAGAC
5′ SOE β-mCherry	GGTGACGACGAGGCAAGGTGCATGGTGAGCAAGGGCGAGGAGG
5′ SOE β-mTFP	GGTGACGACGAGGCAAGGTGCATGGTGAGCAAGGGCGAGGAGAC

Restriction endonuclease cleavage sites used for cloning are shown in small form letters

ISSN: 1475-2859