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Table 1 Killer activity of K28 variants produced by P. pastoris fermentation

From: Production of fluorescent and cytotoxic K28 killer toxin variants through high cell density fermentation of recombinant Pichia pastoris

Toxin variant/control

Toxicity

K28wt

+

K28-mCherryHDEL

+

K28-mCherryΔ

K28-mTFPHDEL

+

mCherryHDEL

mCherryΔ

mTFPHDEL

  1. K28 variants which show killing activity in an agar diffusion assay on methylene blue agar plates in form of a zone of growth inhibition (mm) are marked with “+”, whereas the absence of a killing zone is displayed by “−”