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Table 2 Orthogonal experiment result of transglycosylation reactions for kojibiose synthesis

From: Cloning and expression of the sucrose phosphorylase gene in Bacillus subtilis and synthesis of kojibiose using the recombinant enzyme

No.

Factors

Transformation efficiency (%)

A

B

C

Bank

1

1

1

1

1

35.30

2

1

2

2

2

34.72

3

1

3

3

3

27.69

4

2

1

2

3

37.80

5

2

2

3

1

34.22

6

2

3

1

2

36.65

7

3

1

3

2

29.35

8

3

2

1

3

33.82

9

3

3

2

1

30.04

K1

0.977

1.025

1.058

0.996

 

K2

1.087

1.028

1.026

1.007

 

K3

0.932

0.944

0.913

0.993

 

k1

0.326

0.342

0.353

0.332

 

k2

0.362

0.343

0.342

0.336

 

k3

0.311

0.315

0.304

0.331

 

R

0.155

0.084

0.145

0.014

 
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Microbial Cell Factories

ISSN: 1475-2859

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