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Table 3 Comparison of Tm, refolding and binding kinetics for EBOV GP binders and their derivatives

From: Selection, characterization, and thermal stabilization of llama single domain antibodies towards Ebola virus glycoprotein

Clone name

Tm (°C)

Refolding (%)

ka (1/Ms)

kd (1/s)

KD (M)

EBOV-GP-G6

72

77

7.0 E+04

1.5E−04

2.0E−09

EBOV-GP-G6-neg+

87

67

3.5E+03

1.5E−04

4.4E−08

EBOV-GP-H7

70

97

1.7E+04

3.0E−04

1.7E−08

EBOV-GP-H7-neg+

87

85

3.5E+04

1.4E−03

4.0E−08

EBOV-GP-A8

57

0

3.1E+04

7.2E−05

2.3E−09

EBOV-GP-A8-neg+

73

0

6.1E+04

5.8E−05

9.5E−10

EBOV-GP-A8-fneg+

74

66

1.3E+05

9.1E−05

6.9E−10

  1. Three of the GP binding sdAbs showing the highest melting temperatures and/or best affinities were subject to mutagenesis to improve their stability. Melting temperature and refolding ability were measured by CD
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Microbial Cell Factories

ISSN: 1475-2859

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