Table 1 Densitometric analysis of dot blot experiments with the supernatants of the adsorption reaction performed with 50 µg xylanase (GH10-XA) and 1 × 1010 spores
From: Conversion of xylan by recyclable spores of Bacillus subtilis displaying thermophilic enzymes
Xylanase source | Amount of sample used | Density (OD/mm2)a | Amount of xylanase (ng)b | Xylanase μg (% total)b |
|---|---|---|---|---|
Purified xylanase (ng) | 1000.0 | 690.77 | NA | NA |
500.0 | 450.37 | NA | NA | |
250.0 | 204.87 | NA | NA | |
125.0 | 176.36 | NA | NA | |
62.5 | 114.49 | NA | NA | |
Unbound (μl) | 10.0 | 1149.80 | 1855.10 | 34.08 ± 2.36 |
5.0 | 587.18 | 892.18 | (68%) | |
2.5 | 312.98 | 406.05 |