Fig. 2

Phagocytosis of the M. marinum mutants by macrophages. Macrophages were incubated with M. marinum strains for 2 h at an MOI of 10. Non-ingested bacteria were extensively washed and killed by gentamycin. Macrophages were lysed with Triton X-100, and then cell lysates were plated onto Middlebrook 7H10 agar supplemented with 10% OADC for CFU determination. The number of ingested bacteria are presented as the mean ± SEM from 6 to 7 separate experiments. Figure describes the difference in phagocytosis between mutants, their complemented counterparts and wild-type M. marinum. Statistical significance: *p ≤ 0.03 (Mann–Whitney U test)