Table 1 Primers used in this study
Primers | Sequences (5′–3′) | Employment |
|---|---|---|
CBH2-1183UF | TGAAACCCCTCACTACTGCCAT | cbh2 overexpression strain construction |
CBH2-2817DR | CTAATGCCTCGGGCTGGGACAACGAAATGGTAGGGTACGGTCAG | cbh2 overexpression strain construction |
EG2-1524UF | GACAAGAAATCGGGTGTTTAGGT | egl2 overexpression strain construction |
EG2-1813DR | CTAATGCCTCGGGCTGGGACAAATAAGAATGCGGCCGCGTGGGCTGGGTAGGGTTTG | egl2 overexpression strain construction |
PyrG-S | CTTCCTAATACCGCCTAGTCAT | PyrG markers amplification |
PyrG-A | AGCCGCTGGTCAATGTTATC | PyrG markers amplification |
DR-S | TTGTCCCAGCCCGAGGCATTAG | PyrG markers amplification |
CBH2-1179UF | ACCCCTCACTACTGCCATTTAT | cbh2 overexpression strain construction |
pyrG-1172DR | TATCAATCTGGGGTAACGGACG | cbh2/egl2 overexpression strain construction |
Y-cbh2-F1 | TTCACCTCCTCTTAGTGCAG | cbh2 overexpression strain verification |
Y-PyrG-R1 | TACGGTCGCATAGCAGTG | cbh2/egl2 overexpression strain verification |
Y-egl2-F1 | GCATATGTCAAATTTGGAGCGG | egl2 overexpression strain verification |
Y1 | GCCAGGGATGCTTGAGTGTA | bgl1 overexpression strain verification |
Y2 | CCCAGCCACAGGACCAAGTATG | bgl1 overexpression strain verification |
real-cbhl-Sl | GGTGGCGTGAGCAAGTATCC | Used for RT-PCR |
real-cbhl-Al | TGTCCTCCAATGCCCGTGTT | Used for RT-PCR |
real-cbh2-S | CTGGTCCAACGCCTTCTTCA | Used for RT-PCR |
real-cbh2-A | GACCCAGACAAACGAATCCAG | Used for RT-PCR |
real-actin-S | CCCAAGTCCAACCGTGAGA | Used for RT-PCR |
real-actin-A | CAATGGCGTGAGGAAGAGC | Used for RT-PCR |
real-egl1-S1 | GGCTCGCTCTACCTGTCTCA | Used for RT-PCR |
real-egl1-A1 | GGGTGCCGTTCCTCCAT | Used for RT-PCR |
real- egl2 -S1 | ACGAGCCTTTGGTCGCAGTT | Used for RT-PCR |
real- egl2-A1 | GGCAGCCCAGGTGTTGATGT | Used for RT-PCR |