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Fig. 9 | Microbial Cell Factories

Fig. 9

From: Production of highly efficient cellulase mixtures by genetically exploiting the potentials of Trichoderma reesei endogenous cellulases for hydrolysis of corncob residues

Fig. 9

Construction of the pyrG-excised strains. a Schematic representation of the forced recombination between the DR repeat regions under 5-FOA positive selection to reobtain uracil auxotrophy. b Colony morphology of the 5-FOA-resistant strains grown on the MM medium without uracil. c Colony morphology of 5-FOA-resistant strains grown on the MM medium with uracil. d PCR confirmation of the absence of the pyrG marker in the genome of the pyrG-excised T. reesei strains. e The CMC plate analysis of the pyrG-excised T. reesei strains

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Microbial Cell Factories

ISSN: 1475-2859

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