Fig. 3

FBA for 1,3-PDO production. a Two-dimensional solution space of 1,3-PDO production and cell growth is presented as a shadowed area. Experimental data from Fig. 2 are plotted as filled circles. b The metabolic flux distributions observed in gene disruption simulation are plotted in solution space. Circles, squares, triangles, and crosses represent four groups of the simulated phenotypes. Circles (36) and squares (231) are groups indicating higher and lower productivity relative to the experimental data, respectively. Triangles (254) and crosses (74) are groups indicating a null value of a specific growth rate and no solution satisfying constraint conditions