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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Process optimization for enhancing production of cis-4-hydroxy-l-proline by engineered Escherichia coli

Fig. 2

Optimizing the expression of l-proline cis-4-hydroxylase in E. coli. a CHOP production with different host cells. SmP4H was expressed in E. coli BL21(DE3) and TransB (DE3) respectively; b CHOP production from the resting cells of E. coli BL21(DE3) carrying different expression plasmids. c SDS-PAGE results for the soluble fractions of cell extracts of E. coli BL21(DE3) carrying different expression plasmids. Con, BL21(DE3); B1, BL21/pACYCDuet-1-SmP4H; B2, BL21/pCDFDuet-1-SmP4H; B3, BL21/pRSFDuet-SmP4H; B4, BL21/pET28a-SmP4H; B5, BL21/pET22b-SmP4H; B6, BL21/pETDuet-1 SmP4H

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Microbial Cell Factories

ISSN: 1475-2859

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