Table 1 Bacterial strains and plasmids used in this study

Strains

 Lysobacter enzymogenes

  OH11

Wild-type strain OH11, Km^R

[24]

CGMCC no. 1978

  OH11 (lesR)

OH11 harboring plasmid pBBR-lesR, Km^R, Gm^R

[18]

  OH11 (pBBR)

OH11 harboring plasmid pBBR1-MCS5, Km^R, Gm^R

[18]

  OH11 (lesR-clp)

OH11 harboring plasmid pBBR-lesR-clp, Km^R, Gm^R

This study

  OH11 (clp)

OH11 harboring plasmid pBBR-clp, Km^R, Gm^R

This study

  Δclp

clp in-frame deletion mutant of strain OH11, Km^R

[15]

  Δclp (lesR)

Mutant Δclp harboring plasmid pBBR1-lesR, Km^R, Gm^R

This study

  Δclp (pBBR)

Mutant Δclp harboring plasmid pBBR1-MCS5, Km^R, Gm^R

[15]

  ΔrpfF

The rpfF in-frame deletion mutant of strain OH11, Km^R

[14]

  ΔrpfF (rpfF)

ΔrpfF harboring plasmid pBBR1-rpfF, Km^R, Gm^R

[14]

  ΔrpfF (pBBR)

ΔrpfF harboring plasmid pBBR1-MCS5, Km^R, Gm^R

[14]

Escherichia coli

 DH5a

F⁻, φ80dlacZ∆M15, ∆(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17(r ⁻ _k , m ⁺ _k ), phoA, supE44, λ ⁻ , thi-1, gyrA96

[14]

Plasmids

 pMD19-T

ColE1 origin, T Simple vector, Amp^R

TaKaRa, Shanghai, China

 pBBR1-MCS5

Broad host range cloning vector, lacZ, Gm^R

[25]

 pBBR-clp

pBBR1-MCS5 with 1.2 kb fragment including clp, Gm^R

[15]

 pBBR-lesR

pBBR1-MCS5 with 1.5 kb fragment including lesR, Gm^R

[18]

 pBBR-lesR-clp

pBBR-lesR harboring a 1.25-kp DNA fragment, which contained the coding region of clp and its native promoter region, Gm^R

This study