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Table 3 Results of the mepA deletion in C. glutamicum ATCC 13032

From: Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system

Plasmid

Element

Results (D/W/T)

Efficiency (%)

pFSC, pFST-mepA1

Cas9 + sgRNA1 + Hdarm (300 bp)

3/9/12

25

pFSC, pFST-mepA2

Cas9 + sgRNA2 + Hdarm (300 bp)

0/12/12

0

pFSC, pFST-mepA3

Cas9 + sgRNA3 + Hdarm (300 bp)

10/2/12

83.3

pFSC, pFST-mepA4

Cas9 + sgRNA4 + Hdarm (300 bp)

4/8/12

33.3

pFSC, pFST-mepA5

Cas9 + sgRNA5 + Hdarm (300 bp)

12/0/12

100

pFSC, pFST-mepA6

Cas9 + sgRNA6 + Hdarm (300 bp)

6/6/12

50

  1. D, number of colonies that harbored gene-deleted cells; W, number of colonies that harbored wild type cells; T, total number of colonies used for PCR screening; Efficiency, probability of deletion events occurring, calculated as D/T * 100%
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Microbial Cell Factories

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