Profiling of Saccharomyces cerevisiae transcription factors for engineering the resistance of yeast to lignocellulose-derived inhibitors in biomass conversion

Table 2 Ethanol productivity and ethanol yield of STB5 and YAP1 deletion mutants and transformants in flask cultivations

Strains, mutants and transformants^a	Inhibitor	Q _16h (g/l/h)^b	q _16h (g/g/h)^b	Y _E/G (g/g)^c
BY4741	N/A^d	0.58 ± 0.01	3.93 ± 0.04	0.463 ± 0.011
	Coniferyl aldehyde	NDTD	NDTD	0.349 ± 0.014
	HMF	0.08 ± 0.03	0.58 ± 0.15	0.222 ± 0.007
M-Y	N/A	0.55 ± 0.04	3.85 ± 0.16	0.437 ± 0.031
	Coniferyl aldehyde	NDTD	NDTD	NDTD
	HMF	0.12 ± 0.02	0.81 ± 0.09	0.182 ± 0.007
M-S	N/A	0.52 ± 0.03	3.62 ± 0.08	0.413 ± 0.021
	Coniferyl aldehyde	NDTD	NDTD	0.375 ± 0.023
	HMF	NDTD	NDTD	NDTD
SIY	N/A	0.54 ± 0.02	3.67 ± 0.08	0.434 ± 0.020
	Coniferyl aldehyde	NDTD	NDTD	NDTD
	HMF	0.11 ± 0.01	0.74 ± 0.01	0.241 ± 0.005
YIS	N/A	0.55 ± 0.01	3.91 ± 0.03	0.440 ± 0.005
	Coniferyl aldehyde	NDTD	NDTD	0.317 ± 0.005
	HMF	0.04 ± 0.01	0.28 ± 0.01	0.115 ± 0.023

NDTD none detected
^aBY4741, BY4741 host strain; M-Y, deletion mutant of YAP1; M-S, deletion mutant of STB5; SIY, STB5 overexpressed in deletion mutant of YAP1; YIS, YAP1 overexpressed in deletion mutant of STB5
^bCalculations based on the results within the first 16 h of fermentation. Q is the volumetric ethanol productivity, and q is the specific ethanol productivity
^c Y _E/G is the ethanol yield on the initial amount of fermentable sugar (glucose). The calculations are based on the maximum ethanol concentrations obtained within 136 h of fermentation
^dN/A not applicable; no inhibitor was added to the culture medium

ISSN: 1475-2859