Table 2 Primers used in the present study (restriction sites are underlined)
Primer | Sequence (5′ → 3′) |
|---|---|
For plasmid construction and verification | |
TUP1-F | CCGCTCGAGATGACTGCCAGCGTTTCGAA |
TUP1-R | CCGCTCGAGTTAATTTGGCGCTATTTTTTTA |
PGK-F | CGCGCATGCTCTAACTGATCTATCCAAAACTGA |
PGK-R | ACGCGCATGCTAACGAACGCAGAATTTTC |
Kan-F | ACATGGATCCCAGCTGAAGCTTCGTACGC |
Kan-R | ACATGGATCCGCATAGGCCACTAGTGGATCTG |
T-F | CCGCTCGAGATGACTGCCAGCGTTTCGAATACG |
T-R | CCGCTCGAGTTAATTTGGCGCTATTTTTTTATACTTCCAAATC |
T-C-F | CTAGTTTGCAC CAGGATCACTACTTAGTCCTCATCGAAATTCGATCACTGAAAATAAC |
T-C-R | ACGTGGTGGTGTTATTTTCAGTGATCGAATTTCGATGAGGGACTAAGTAGTGATCCGTG |
T-B-F | CTAACTCACAGGAAAATGAAGGACGCGCCCCAGGTTTCCGTGGCA |
T-B-R | AATGGTGCCACGGAAACCTGGGGCGCGTCCTTCATTTTCCTGTGA |
T-A-F | CCGCTCGAGATGTACGAAGAAGAGATCAAGCACTTGAAAC |
T-A-R | CCGCTCGAGTTAATTTGGCGCTATTTTTTTATACTTCCAAATC |
T-D-F | CCGCTCGAGATGCATCGAAATTCGATCACTGAAAA |
T-D-R | CCGCTCGAGTTAATTTGGCGCTATTTTTTTATAC |
T-E-F | CCGCTCGAGATGACTGCCAGCGTTTCGAA |
T-E-R | CCGCTCGAGTTAGTTATTGGCAGCAGAATCGTCAGA |
For real-time PCR | |
MAL62-F | AGTTTCCTGGCAAATCGG |
MAL62-R | GTCCCACGGCAATCATAC |
MAL61-F | TACCTCCGTTTGTTTGCG |
MAL61-R | AGGACCATTGTGAGACCC |
ACT1-F | ATTGATAACGGTTCTGGT |
ACT1-R | AATTGGGTAACGTAAAGTC |