Fig. 2
From: Structure-based antigenic epitope and PEGylation improve the efficacy of staphylokinase
Preparation of Sak and its PEGylation mutants. a The Sak-cys protein (Sak-C79G) was purified by Ni2+-NTA affinity chromatography. Lane 1: protein marker; lane 2: pellet; lane 3: supernatant; lane 4: flow through; lane 5: elution with washing buffer; lane 6: elution with elution buffer. b Size exclusion gel filtration chromatography of Sak using the Superdex 200 column. c Figures with Coomassie blue staining (upper part) and identification of iodine staining (lower part) are combined together. Lane 1: protein marker; lane 2: reaction mixtures; lane 3: elution of peak 1; lane4: elution of peak 2; lane 5: elution of peak 3. d SDS-PAGE analysis of the PEG-Sak proteins. Lane 1: protein marker; lane 2: PEG-Sak-C79G; lane 3: PEG-Sak-C82L; lane 4: PEG-Sak-C84K; lane 5: PEG-Sak-C97A; lane 6: PEG-Sak-C104R