Fig. 4
From: The expression and construction of engineering Escherichia coli producing humanized AluY RNAs
Effects of digestion of DNA enzyme and RNA enzyme. a The result of northern detection. pET-AluY × 8 DE3 bacteria were induced with IPTG (final concentration 0.2 mg/ml) at 37 °C for 4 h. RNAs were extracted using SDS-hot phenol method and digested with DNase I (lane 1), without DNase I (lane 2), or with DNase I plus RNase A (lane 3). AluY RNAs were detected using northern blotting. b Dyeing results of methylene blue; c IOD values of each lane of the northern blotting results (means of three independent experiments). “*”, the AluY RNA yield of pET-AluY × 8 DNase I digestion and pET-AluY × 8 without DNase I digestion was significantly higher than that of pET-AluY × 8 DNase I plus RNase A digestion (p < 0.01)