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Table 3 Intracellular NADH oxidase activities, NAD (H) concentrations and ratio of NAD+ to NADH in MNR M3, MNR M3N1 and MNR M3N2

From: Cofactor engineering to regulate NAD+/NADH ratio with its application to phytosterols biotransformation

Strains

MNR M3

MNR M3N1

MNR M3N2

NADH oxidase activity (U/mg)

ND

0.32 ± 0.02

0.86 ± 0.02

NAD+ (μmol/g DCW)

9.34 ± 0.50

9.71 ± 0.33

9.11 ± 0.72

NADH (μmol/g DCW)

0.90 ± 0.15

0.44 ± 0.05

0.30 ± 0.02

Ratio of NAD+ to NADH

10.4

22.1

30.4

  1. Biotransformation conditions: 50 mL fermentation medium in 250 mL shake flask, 30 °C, 140 r/min, 96 h. All experiments were performed in triplicate
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Microbial Cell Factories

ISSN: 1475-2859

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