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Table 1 Six digesters were screened cytometrically

From: Flow cytometric quantification, sorting and sequencing of methanogenic archaea based on F420 autofluorescence

Digester

Substrate

Process parameters

F420+

HRT [day]

OLR [g L−1 day−1]

Temp. [°C]

Stirring [min−1]

Cell number [mL−1] (± standard dev.)

Autofluorescence intensity (± standard dev.)

A

Disintegrated straw

60

2.5

40

100

1.24 × 109 (± 4.98 × 108)

33.57 (± 0.85)

B

Whole-plant rye silage

40

2.5

40

200

4.50 × 108 (± 6.85 × 106)

40.20 (± 2.31)

C

Corn silage

150

2.5

40

100

3.60 × 109 (± 1.46 × 108)

21.90 (± 0.56)

D

Chicken manure

60

2

30

100

2.40 × 109 (± 2.04 × 108)

38.13 (± 5.89)

E

Chicken manure

60

2

39

100

9.14 × 108 (± 4.66 × 107)

35.70 (± 3.22)

F

Common duckweed

40

1

40

70

6.17 × 107 (± 2.42 × 106)

33.03 (± 2.34)

G

Elodea nuttallii

285

2.7

37

160

1.02 × 109 (± 1.80 × 108)

26.37 (± 0.50)

H

Synthetic organic acids

8

4.65

37

50

2.23 × 109 (± 8.60 × 107)

48.73 (± 0.49)

  1. The screened digesters differed in substrate input and their main process parameters. Cell numbers and mean intensities of the autofluorescent subcommunities F420+ are given with standard deviations