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Table 1 Six digesters were screened cytometrically

From: Flow cytometric quantification, sorting and sequencing of methanogenic archaea based on F420 autofluorescence

Digester Substrate Process parameters F420+
HRT [day] OLR [g L−1 day−1] Temp. [°C] Stirring [min−1] Cell number [mL−1] (± standard dev.) Autofluorescence intensity (± standard dev.)
A Disintegrated straw 60 2.5 40 100 1.24 × 109 (± 4.98 × 108) 33.57 (± 0.85)
B Whole-plant rye silage 40 2.5 40 200 4.50 × 108 (± 6.85 × 106) 40.20 (± 2.31)
C Corn silage 150 2.5 40 100 3.60 × 109 (± 1.46 × 108) 21.90 (± 0.56)
D Chicken manure 60 2 30 100 2.40 × 109 (± 2.04 × 108) 38.13 (± 5.89)
E Chicken manure 60 2 39 100 9.14 × 108 (± 4.66 × 107) 35.70 (± 3.22)
F Common duckweed 40 1 40 70 6.17 × 107 (± 2.42 × 106) 33.03 (± 2.34)
G Elodea nuttallii 285 2.7 37 160 1.02 × 109 (± 1.80 × 108) 26.37 (± 0.50)
H Synthetic organic acids 8 4.65 37 50 2.23 × 109 (± 8.60 × 107) 48.73 (± 0.49)
  1. The screened digesters differed in substrate input and their main process parameters. Cell numbers and mean intensities of the autofluorescent subcommunities F420+ are given with standard deviations