Fig. 3

Production of soluble full-length bank vole PrP. a Coomassie blue staining of SDS-PAGE gel of full-length bank vole PrP 109M purified after Ni–NTA: lanes 1–9 are the elution peaks and lane 10 is the molecular marker. b Size exclusion chromatography (SEC) of full-length rBVPrP-109M using superdex 75 HR1030 column. c Coomassie blue staining of SDS-PAGE gel of purified full-length rBVPrP-109M after SEC. d Coomassie blue staining of the purified full-length bank vole PrP 109M and 109I after dialysis with 10 mM NaAC pH 4.6. e Western blotting of purified rBVPrP probed with the anti-His-tag antibody as in d