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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Soluble polymorphic bank vole prion proteins induced by co-expression of quiescin sulfhydryl oxidase in E. coli and their aggregation behaviors

Fig. 3

Production of soluble full-length bank vole PrP. a Coomassie blue staining of SDS-PAGE gel of full-length bank vole PrP 109M purified after Ni–NTA: lanes 1–9 are the elution peaks and lane 10 is the molecular marker. b Size exclusion chromatography (SEC) of full-length rBVPrP-109M using superdex 75 HR1030 column. c Coomassie blue staining of SDS-PAGE gel of purified full-length rBVPrP-109M after SEC. d Coomassie blue staining of the purified full-length bank vole PrP 109M and 109I after dialysis with 10 mM NaAC pH 4.6. e Western blotting of purified rBVPrP probed with the anti-His-tag antibody as in d

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