Table 2 Plasmids used

pGM160

E. coli/Streptomyces shuttle vector. Thiostrepton and gentamicin resistance

[44]

pGM160-YefMsl

pGM160 derivative. The xysA promoter from S. halstedii controls yefMsl expression

[21]

pN702Gem3

E. coli/Streptomyces shuttle vector. Neomycin resistance. High-copy number

[37]

pN702Gem3-Tox

pN702GEM3 derivative. The xysA promoter from S. halstedii controls toxin expression

[21]

pTES

E. coli/Streptomyces shuttle vector. Apramycin resistance. Integrative plasmid. attP flanked by loxP sites

[33]

pTES-Tox

pTES derivative. The xysA promoter from S. halstedii controls toxin expression

This work

pXHis1

pBluescript SK derivative. Ampicillin resistance. The xysA promoter from S. halstedii controls xylanase expression

[41]

pXHis1-Anti-T

pXHis1 derivative. The xysA promoter from S. halstedii controls antitoxin expression

This work

pTOS

E. coli/Streptomyces shuttle vector. Apramycin resistance. Integrative plasmid. attP flanked by rox sites

[33]

pTOS-Neo

pTOS derivative. The neomycin resistance gene is flanked by rox sites

This work

pN702Gem3Rox

pN702Gem3 derivative. The neomycin resistance gene is flanked by rox sites

This work

pN702Gem3Rox-Anti-T

pN702Gem3Rox derivative. The xysA promoter from S. halstedii controls antitoxin expression

This work

pNUF5

pN702Gem3 derivative. The pstS promoter from S. lividans controls xylanase expression

[36]

pNUF-Amy

pNUF5 derivative. The pstS promoter from S. lividans controls amylase expression

[22]

pNRoxAnti-Xyl

pN702Gem3Rox-Anti-T derivative. The xysA promoter from S. halstedii controls antitoxin expression and the pstS promoter from S. lividans controls xylanase expression

This work

pNRoxAnti-Amy

pN702Gem3Rox-Anti-T derivative. The xysA promoter from S. halstedii controls antitoxin expression and the pstS promoter from S. lividans controls amylase expression

This work

pRoxAnti-Xyl

pNRoxAnti-Xyl derivative without neomycin resistance gene

This work

pRoxAnti-Amy

pNRoxAnti-Amy derivative without neomycin resistance gene

This work