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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Use of the KlADH3 promoter for the quantitative production of the murine PDE5A isoforms in the yeast Kluyveromyces lactis

Fig. 7

Coomassie-stained SDS-PAGE analysis of the 3XFlagPDE5 isoforms purification processes. The figure reports the whole protein extracts (WE), the flow-through (FT) and the eluted fractions (I, II and III) of the A1 (lanes 1–8) and A2 (lanes 9–16) isoforms purified by an ANTI-FLAG M2 affinity chromatography column. The figure also reports the different MW of the three purified isoforms (lanes 17–19). WE was from 100 mL of YPG cultures grown for 4 days. BSA standards were used for the quantification

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Microbial Cell Factories

ISSN: 1475-2859

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