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Table 2 Real-time PCR primers of key genes involved in polysaccharides synthesis

From: Increasing the bioflocculant production and identifying the effect of overexpressing epsB on the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis

Name

Primer sequences (5′–3′)

q-crr-F

TCGGCATCCGTTCACTGT

q-crr-R

CCAAATCGCACGTTATCAAA

q-pgcA-F

AATGGAAGCTGCTAAAACGC

q-pgcA-R

AAAGGAAAGTTCAGGTGTCGG

q-gtaB1-F

GCAAAAGGAGCCCCTCGGTC

q-gtaB1-R

CGAGAAGGACGGCAAACGG

q-epsA-F

CAATACACGCGGACATTCCA

q-epsA-R

CTCTGATTCGCTTTCACTGCTC

q-epsB-F

ATCCTTTGAGAAGCCGTTTA

q-epsB-R

CCAAGTTTGAAGCCGAGAA