Table 1 Plasmids and strains used in this study
From: Biosynthesis and engineering of kaempferol in Saccharomyces cerevisiae
Plasmid or strain | Genotype | Source or reference |
|---|---|---|
Plasmids | ||
 YCplac22 | Amp Trp1 | Gietz [50] |
 YCplac33 | AmpURA3 | Gietz [50] |
 Y22-T4 | T ADH1 /T TDH3 | This work |
 Y22-AtF3H | P PGK1 -AtF3H-T ADH1 | This work |
 Y22-T4-AtF3H-AtFLS | P PGK1 -AtF3H-T ADH1 /P TDH3 -AtFLS-T TDH1 | This work |
 Y22-T4-AtF3H-CitFLS | P PGK1 -AtF3H-T ADH1 /P TDH3 -CitFLS-T TDH1 | This work |
 Y22-T4-AtF3H-MdFLS | P PGK1 -AtF3H-T ADH1 /P TDH3 -MdFLS-T TDH1 | This work |
 Y22-T4-AtF3H-PdFLS | P PGK1 -AtF3H-T ADH1 /P TDH3 -PdFLS-T TDH1 | This work |
 Y22-T4-AtF3H-ZmFLS | P PGK1 -AtF3H-T ADH1 /P TDH3 -ZmFLS-T TDH1 | This work |
 Y33-ALAC-ADH2 | P TEF2 -ACS SE-T RPS2 /P PGK1 -ALD6-T TDH1 /P HXT7 -ADH2-T RPL9A | This work |
 Y33-ALAC-ACAD | P TEF2 -ACS SE-T RPS2 /P PGK1 -ALD6-T TDH1 /P HXT7 -ADH2-T RPL9A /P PGK1 -ACC1-T CCW12 | This work |
Strains | ||
 W303-1A | MATa leu2-3112 ura3-1 trp1-92 his3-11,15 ade2-1 can1-100 | Thomas [51] |
 W3-AtF3H | W303-1A carrying Y22-AtF3H | This work |
 W3-AtFLS | W303-1A carrying Y22-T4-AtF3H-AtFLS | This work |
 W3-CitFLS | W303-1A carrying Y22-T4-AtF3H-CitFLS | This work |
 W3-MdFLS | W303-1A carrying Y22-T4-AtF3H-MdFLS | This work |
 W3-PdFLS | W303-1A carrying Y22-T4-AtF3H-PdFLS | This work |
 W3-ZmFLS | W303-1A carrying Y22-T4-AtF3H-ZmFLS | This work |
 W3NP | YORWdelta17::His3/P ADH1 -4CL-T TPI1 /P HXT7 -CHS-T TPG1 /P PGI1 -CHI-T ADH1 /P ADH1 -PAL-T FBA1 /P TDH3 -C4H-T PDC1 | This work |
 W3NP-FF | W3NP carrying Y22-T4-AtF3H-PdFLS and YCplac33 | This work |
 W3NP-FF-A3 | W3NP carrying Y22-T4-AtF3H-PdFLS and Y33-ALAC-ADH2 | This work |
 W3NP-FF-A4 | W3NP carrying Y22-T4-AtF3H-PdFLS and Y33-ALAC-ACAD | This work |