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Fig. 5 | Microbial Cell Factories

Fig. 5

From: The combination of recombinant and non-recombinant Bacillus subtilis spore display technology for presentation of antigen and adjuvant on single spore

Fig. 5

Detection of native FliD of Clostridium difficile 630 by sera of immunized animals. Flagellar preparations were fractionated on SDS-PAGE and analyzed by Western blotting with pooled sera of each group of immunized animals. The lines 3–7 correspond to groups of animals administered with: (3) purified FliD, (4) empty 168 spores, (5) 168/FliD spores, (6) empty BKH121 spores, (7) BKH121/FliD spores. (1) Molecular mass marker, (2) the naïve group

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