Fig. 2

Spore surface display of adsorbed FliD protein as assessed by immunofluorescence microscopy. Purified, free spores of wild-type strain 168 and recombinant BKH121 alone and with adsorption of FliD protein (168/FliD, BKH121/FliD) were visualized by phase contrast and immunofluorescence microscopy. The spores were incubated with mouse anti-FliD antibodies, followed by anti-mouse IgG–Cy3 conjugates. The same exposure time was used for all immunofluorescence images. Scale bar—10 μm