Effect of cell permeability and dehydrogenase expression on octane activation by CYP153A6-based whole cell Escherichia coli catalysts

Table 3 Change in product formation rates over 24 h of biotransformation

Culture description	Initial rate^a (mmol L⁻¹ min⁻¹)	Final rate^b (mmol L⁻¹ min⁻¹)	Rate loss over 24 h (%)
Whole cells −GLD	0.0348 ± 0.0020	0.0140 ± 0.0017	60
Whole cells +GLD	0.0325 ± 0.0010	0.0036 ± 0.0001	89
Rupture −GLD	0.0185 ± 0.0001	0.0071 ± 0.0099	62
Rupture +GLD	0.0290 ± 0.0003	0.0175 ± 0.0015	40
Disintegration −GLD	0.0131 ± 0.0007	0.0058 ± 0.0006	56
Disintegration +GLD	0.0232 ± 0.0013	0.0151 ± 0.0050	35
Cell free extract −GLD	0.0054 ± 0.0003	0.0028 ± 0.0001	48
Cell free extract +GLD	0.0089 ± 0.0009	0.0106 ± 0.0013	0
Acetone +GLD	0.0187 ± 0.0010	0.0084 ± 0.0011	55
Polymyxin B +GLD	0.0626 ± 0.0009	0.0034 ± 0.0016	95
Triton X-100 +GLD	0.1060 ± 0.0015	0.0002 ± 0.0006	100

Measurements were taken at five time points over the first 24 h of biotransformation. Two vials were sacrificed at each time point for sampling. The organic phase was extracted into ethyl acetate and analysed via gas chromatography. Rates are based on the combined concentrations of 1-octanol and octyl acetate, which were the only significant products observed
^aCalculated between 0 and 4 h of biotransformation
^bCalculated between 10 and 24 h of biotransformation

ISSN: 1475-2859