Fig. 3

Effect of additional GLD expression on product formation over 24 h, using chemically permeabilised cells expressing additional GLD. Whole cells without additional GLD are included for comparison. Cultures were at a high cell density, and produced octyl acetate by-product alongside 1-octanol (an artefact of the expression system). The product concentrations shown here are the combined concentrations of 1-octanol and octyl acetate. Two vials were sacrificed to obtain each sample point. The organic phase was extracted into ethyl acetate and analysed via gas chromatography