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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica

Fig. 7

Quantification of eGFP expressed in transgenic T. pseudonana (a) and C. cryptica (b) by western blot. PVDF membrane containing transferred GFP standard and total soluble proteins were probed with anti-GFP monoclonal antibodies. BioRad’s ChemiDoc imaging system and Image Lab software were used to detect and quantify chemiluminescent signals from the blot. Numbers indicate ng of eGFP and µg of TSP loaded each well. Arrow heads indicate recombinant eGFP. The lower band is a native T. pseudonana protein with peroxidase activity. eGFP in a shift reflects the fused N-terminal SIT sequence

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Microbial Cell Factories

ISSN: 1475-2859

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