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Fig. 5 | Microbial Cell Factories

Fig. 5

From: In vivo plug-and-play: a modular multi-enzyme single-cell catalyst for the asymmetric amination of ketoacids and ketones

Fig. 5

Fermentative amination of α-ketoacids with enzyme-coupled NADH regeneration. Fermentative reductive amination of KMV to l-isoleucine with Ec-AlaDH carrying pTrc99A-avtA (catalyst 3a, 4a, 5a) or pTrc99A-ald-avtA (catalyst 3b, 4b, 5b) and using either FDH (a), GDH (b) or PtDH (c) for cofactor regeneration. Strains were inoculated in SGA medium with OD600 4 and 100 mM co-substrate, 50 mM (NH4)2SO4 and 0–250 mM l-alanine were added to push the reaction equilibrium towards product side

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Microbial Cell Factories

ISSN: 1475-2859

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