Table 3 Transformation efficiency of the plasmids studied for L. starkeyi
Plasmid | Signal peptide | Cellulase gene | DNA used for transformation (mg DNA) | No. of colonies | No. of colonies/mg DNA |
|---|---|---|---|---|---|
pLS1 | spPRO | eg2 | 11.6 | 66 | 6 |
pLS4 | spPRO | TeTrcbh1 | 23.6 | 1024 | 43 |
pLS2 | spAMY | eg2 | 4.9 | 44 | 9 |
pLS5 | spAMY | TeTrcbh1 | 37.5 | 127 | 3 |
pLS3 | spDEX1 | eg2 | 17.5 | 154 | 9 |
pLS6 | spDEX1 | TeTrcbh1 | 17.9 | 48 | 3 |
pLS7 | spDEX2 | eg2 | 23.5 | 1920 | 82 |
pLS8 | spDEX2 | TeTrcbh1 | 6.5 | 22 | 3 |