Expression and secretion of fungal endoglucanase II and chimeric cellobiohydrolase I in the oleaginous yeast Lipomyces starkeyi

Table 2 Signal peptides used in this study and their guided recombinant proteins

Signal peptide^a	Gene accession no.	Sequence of signal peptide^b	No. of residues	Constructed plasmid and secretion of cellulases^c
Signal peptide^a	Gene accession no.	Sequence of signal peptide^b	No. of residues	EG II	Chimeric TeTrCBH I
Protease (spPRO)	P09230	MKLATAFTILTAVLA/APL	15	pLS1 (EG II); Y	pLS 4 (CBH I); Y
Amylase (spAMY)	AY155463	MLLINFFIAVLGVISLSPIVVARYILRR/DCT	28	pLS2 (EG II); Y	pLS 5 (CBH I); Y
Dextranase 1 (spDEX1)	AY520537	MTLIYVPSIFTMVPSITRIVLVNILLATLVLG/AAV	32	pLS3 (EG II); Y	pLS 6 (CBH I); ND
Dextranase 2 (spDEX2)	AAS90631	MVPSITRIVLVNILLATLVLG/AAV	21	pLS7 (EG II); Y	pLS 8 (CBH I); Y

^aExcept the signal peptide of protease (spPRO) that was derived from Y. lipolytica, all other signal peptides were derived from L. starkeyi
^b/, cleavage site for a signal peptidase between the signal peptide and the mature protein
^cFor the secretion of cellulases in the cells transformed with the constructed plasmids, Y represents efficient secretion, whereas ND for not detected

ISSN: 1475-2859