Fig. 4
From: Systematic evaluation of characteristics of the membrane-based fed-batch shake flask
Distribution of ammonium and phosphate in the culture broth and the reservoir over time in E. coli fed-batch cultivations. a, c Ammonium amount for three initial reservoir glucose concentrations (250, 375 and 500 g/L). b, d Phosphate amount for three initial reservoir glucose concentrations (250, 375 and 500 g/L); cultivation conditions: Wilms-MOPS-mineral medium (0.2 M MOPS), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, culture broth volume: 10 mL, inoculation OD600: 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt (1) dialysis membrane, membrane area: 18.1 mm2; strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP