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Table 3 Process parameters for galactaric acid production at 0.5, 1, 10 and 250 L

From: Scaling up and scaling down the production of galactaric acid from pectin using Trichoderma reesei

  d-Galacturonic acida Meridianstar pectin Sigma pectin Meridianstar pectin
Reactor volume (L) 0.5 1 10 250
Process time (h) 242 215 185 168
Inputs
 Lactose (g L−1) 28 10 14 9
 Galacturonic acid (g L−1) 21    
 Pectin (g L−1)   48.4 29.2 45.9
 Pectinase(s) (mL L−1)   1 1 2
 Fungal cells (g L−1) 1 1 1 1
 Air (vvm) 1.4 0.5 0.5 0.5
Output
 Galactaric acid (g L−1) 21 ± 2 18 21 15
 Yield (g [g galacturonate]−1) 1.0 ± 0.2 1.0 1.1 0.8
 Yield (g [g pectin]−1)   0.41 0.73 0.32
 Biomass (g L−1)b 4 ± 1 9 ± 0.1 4 ± 0.1 8 ± 0.2
 Product recovery (kg)   0.014 0.15 2.8
 Product recovery (%) 68 ± 7 89 85 73
  1. aData from [7]. Values are mean ± sem for four cultivations
  2. bBiomass measurements are mean ± sem for 2–3 replicates, except for 0.5 L cultivations (mean ± sem for 4 cultivations), at end of cultivation