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Table 3 Process parameters for galactaric acid production at 0.5, 1, 10 and 250 L

From: Scaling up and scaling down the production of galactaric acid from pectin using Trichoderma reesei

 

d-Galacturonic acida

Meridianstar pectin

Sigma pectin

Meridianstar pectin

Reactor volume (L)

0.5

1

10

250

Process time (h)

242

215

185

168

Inputs

 Lactose (g L−1)

28

10

14

9

 Galacturonic acid (g L−1)

21

   

 Pectin (g L−1)

 

48.4

29.2

45.9

 Pectinase(s) (mL L−1)

 

1

1

2

 Fungal cells (g L−1)

1

1

1

1

 Air (vvm)

1.4

0.5

0.5

0.5

Output

 Galactaric acid (g L−1)

21 ± 2

18

21

15

 Yield (g [g galacturonate]−1)

1.0 ± 0.2

1.0

1.1

0.8

 Yield (g [g pectin]−1)

 

0.41

0.73

0.32

 Biomass (g L−1)b

4 ± 1

9 ± 0.1

4 ± 0.1

8 ± 0.2

 Product recovery (kg)

 

0.014

0.15

2.8

 Product recovery (%)

68 ± 7

89

85

73

  1. aData from [7]. Values are mean ± sem for four cultivations
  2. bBiomass measurements are mean ± sem for 2–3 replicates, except for 0.5 L cultivations (mean ± sem for 4 cultivations), at end of cultivation