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Table 1 Galactaric acid production by T. reesei expressing udh under promoters pCBH1, pcDNA1, pPDC1 or pGPDA

From: Scaling up and scaling down the production of galactaric acid from pectin using Trichoderma reesei

Strain pCBH1 pcDNA1 pPDC1 pGPDA a
24-well plates—d-galacturonate
 Galactaric acid (g L−1) 11.1 ± 0.2 9.4 ± 0.4 9.8 ± 0.1 8.8
 Galactaric acid production rate (72–120 h) (g l−1 h−1) 0.13 ± 0.01 0.12 ± 0.00 0.12 ± 0.00 0.11
 Yield galactaric/d-galacturonic (g g−1) 0.95 ± 0.02 0.87 ± 0.02 0.89 ± 0.00 0.89
24-well plates—pectin
 Galactaric acid (g L−1) 11.6 ± 0.2 11.5 ± 0.3 11.4 ± 0.1 10.5
 Galactaric acid production rate (72–120 h) (g L−1 h−1) 0.12 ± 0.04 0.11 ± 0.00 0.11 ± 0.00 0.13
 Yield galactaric/d-galacturonic (g g−1) 0.84 ± 0.03 0.90 ± 0.03 0.89 ± 0.03 0.76
0.5 L bioreactors—d-galacturonate
 Galactaric acid (g L−1) 15.7 13.6 16.0 19.7
 Galactaric acid production rate (0–72 h) (g L−1 h−1) 0.22 0.21 0.09 0.19
 Yield galactaric/d-galacturonic (g g−1) 0.90 0.82 1.11 0.85
 Maximum biomass (g L−1) 9.5 ± 0.2 9.0 ± 0.3 6.5 ± 0.5 7.9 ± 0.3
  1. Galactaric acid production (titre, rate of production and yield on d-galacturonic acid) in 24-well plates and in 0.5 L bioreactors using either d-galacturonic acid or pectin (Sigma) as substrate, with lactose as co-substrate. Maximum biomass in bioreactors was observed at 50 (pCBH1, pcDNA1, pGPDA) or 72 (pPDC1) h after which time biomass decreases (see also Fig. 3). Values are mean ± sem for three replicates
  2. aStrain VTT D-161646