Table 1 Strains and plasmids used in this study
Strain | Genotype or plasmid | Source |
|---|---|---|
E. coli | ||
DH5α | F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK-mK+), λ– | [52] |
DH5α | pAD-YlER | This study |
DH5α | pAD-YlERhis | This study |
Y. lipolytica | ||
AJD | MATA, A101: ura3-302 | [29] |
AMM | MATA, MK1: ura3-302 | [29] |
MK1 | MATA, UV-mutant | [19] |
AJD pAD- YlERhis | MATA, A101: ura3-302, pAD-YlER | This study |
AMM pAD- YlER | MATA, A101: ura3-302, pADUTGut2 | This study |