Table 2 Oligonucleotide primers used in this study
From: Development of a counterselectable seamless mutagenesis system in lactic acid bacteria
Primer | Sequence (5′–3′)a | Restriction site |
|---|---|---|
aldB-uF | AGGGTACCGGCGAAAGTCATGTAACAATCC | KpnI |
aldB-uR | CTGACATGATATTTCTCTTTTCTAT | Â |
aldB-dR | CCGCTCGAGTGCTGACAGATGGCTGGCTGTG | XhoI |
aldB-dF | GAAAAGAGAAATATCATGTCAGTAATTGCTTAAATTTCTTTAGC | Â |
aldB-testF | ATATTTCTGCCACAATTTTCATGCC | Â |
aldB-testR | CCAATCCTGTACCAATAACAGCAAT | Â |
pheSF | ATAAAAAATCGAAAAGGAGATAAAAATGAACTTACAAGAAAAAATTGAAG | Â |
pheSR | AAAGATCTTCAGTCGAATTGTTCTAAGAATC | BglII |
pheSR2 | AAGAATTCTCAGTCGAATTGTTCTAAGAATC | EcoRI |
siteR | ACCAAAACCAGAATAAACAGAAG | Â |
siteF | CTGTTTATTCTGGTTTTGGTTTTGGACTCGGTCAAGAACG | Â |
ldhF1 | CCGAATTCATTCATTTTACACATTGTA | EcoRI |
ldhF2 | GACAGATCTATTCATTTTACACATTGTA | BglII |
ldhF3 | AGACGTCGACATTCATTTTACACATTGTA | SalI |
ldhR1 | TTTTATCTCCTTTTCGATTTTTTAT | Â |
ldhR2 | CGGGATCCTTTTATCTCCTTTTCGATTTTTTAT | BamHI |
ldhR3 | CCGCTCGAGTTTTATCTCCTTTTCGATTTTTTAT | XhoI |
upF | AGGGTACCATGTCAATAGTTGTCGAAAA | KpnI |
upR | CAGTTTCTGCTAAGGTATCA | Â |
downF | TGATACCTTAGCAGAAACTGATGAATTAGCACAGCAAGTG | Â |
downR | CCGCTCGAGCTCTAGTAAAATGTTCCTCA | XhoI |
testF | TTAAGGAAATGAATTTAGAGGAGAG | Â |
testR | AAACCTTCATGTCCTTCTTGAGT | Â |
BL-pheSF | AAAGATCTATGGATCTTCAAACCAAACTTG | BglII |
BL-siteR | ACCAAAACCGCCGTAAACGTC | Â |
BL-siteF | GACGTTTACGGCGGTTTTGGTTTTGGCCTTGGTCCTGATCG | Â |
BL-pheSR | GATCTGCAGTTAACCCTCCTGGCTGAATTGC | PstI |
gfpF | ATAAAAAATCGAAAAGGAGATAAAAAGATATGAGCAAAGGAG | Â |
gfpR | CGCGAATTCTTAGTAGAGCTCATC | EcoRI |