Table 1 Plasmids and bacterial strains used in this study
From: Development of a counterselectable seamless mutagenesis system in lactic acid bacteria
| Strain or plasmid | Characteristic(s) | Source |
|---|---|---|
| Strains | ||
| Escherichia coli DH5α | F− supE44 ∆lacU169 Ф80lacZ ∆M15 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Novagen |
| Lactococcus lactis strains | ||
| NZ9000 | Derivative of MG1363, pepN::nisRK | [33] |
| IGn | Derivative of NZ9000, galK::pG+UD-nP-pheS* | This work |
| dA | Derivative of NZ9000, ∆aldB | This work |
| Lactobacillus casei BL23 | Derivative of L. casei ATCC 393 (pLZ15−) | [36] |
| Plasmids | ||
| pG+host9 | Ermr; temperature-sensitive vector | [16] |
| pG+UD | pG+host9 derivative with up and down homology arms of the part of galactose operon | This work |
| pG+UD-nP-pheS* | pG+UD derivative with pheS* driven by n Pldh | This work |
| pG+-nP-pheS* | pG+host9 derivative with pheS* driven by n Pldh | This work |
| pG+UD2-5P-pheS* | pG+-5P-pheS* derivative with upstream and downstream sequences of the aldB gene | This work |
| pUC19 | Ampr; cloing vector | This work |
| pSec:Leiss:Nuc | pWV01 replicon; expresses Nuc under PnisA control; Cmr | [34] |
| pleiss-P-pheS* | pSec:Leiss:Nuc derivative with pheS* driven by a Pldh | This work |
| pTRKH2 | Ermr; expressing vector | [37] |
| pOgfp | Source of gfp gene | [18] |