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Table 1 Plasmids and bacterial strains used in this study

From: Development of a counterselectable seamless mutagenesis system in lactic acid bacteria

Strain or plasmid Characteristic(s) Source
Strains
 Escherichia coli DH5α F supE44 ∆lacU169 Ф80lacZ ∆M15 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Novagen
 Lactococcus lactis strains
  NZ9000 Derivative of MG1363, pepN::nisRK [33]
  IGn Derivative of NZ9000, galK::pG+UD-nP-pheS* This work
  dA Derivative of NZ9000, ∆aldB This work
 Lactobacillus casei BL23 Derivative of L. casei ATCC 393 (pLZ15) [36]
 Plasmids
  pG+host9 Ermr; temperature-sensitive vector [16]
  pG+UD pG+host9 derivative with up and down homology arms of the part of galactose operon This work
  pG+UD-nP-pheS* pG+UD derivative with pheS* driven by n Pldh This work
  pG+-nP-pheS* pG+host9 derivative with pheS* driven by n Pldh This work
  pG+UD2-5P-pheS* pG+-5P-pheS* derivative with upstream and downstream sequences of the aldB gene This work
  pUC19 Ampr; cloing vector This work
  pSec:Leiss:Nuc pWV01 replicon; expresses Nuc under PnisA control; Cmr [34]
  pleiss-P-pheS* pSec:Leiss:Nuc derivative with pheS* driven by a Pldh This work
  pTRKH2 Ermr; expressing vector [37]
  pOgfp Source of gfp gene [18]