Fig. 2From: Development of a counterselectable seamless mutagenesis system in lactic acid bacteriaConstruction of a vector for detecting the PheS*/pG+host9 counterselectable system. The wild-type pheS was changed to pheS* using an overlap extension PCR to introduce a point mutation of GCT to GGT. Pldh: the promoter region of the l-lactate dehydrogenase gene (ldh) (accession number: NC_017949) from Lc. lactis NZ9000Back to article page