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Table 1 The fatty acid composition in DELTA-12 and DELTA-6 desaturase genes overexpressing strains

From: Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases

Strains

Time (h)

Fatty acid composition (relative %, w/w)a

16:0

18:0

18:1

18:2 (LA)

18:3 (GLA)

Mc-1552

 

24

23.1 ± 0.5

4.0 ± 2.0

23.2 ± 8.7

17.4 ± 5.5

30.0 ± 7.1

 

36

23.8 ± 0.5

3.6 ± 0.1

26.2 ± 0.1

14.8 ± 1.6

31.0 ± 1.2

 

48

21.1 ± 0.2

2.7 ± 1.3

28.3 ± 2.6

14.5 ± 1.3

31.1 ± 2.6

 

60

18.2 ± 0.9

2.2 ± 0.6

24.6 ± 0.2

14.3 ± 1.8

31.2 ± 0.1

 

72

17.1 ± 0.6

2.0 ± 0.5

24.0 ± 3.6

14.3 ± 3.2

31.2 ± 0.4

Mc-D12

 

24

23.0 ± 8.4

3.0 ± 0.9

12.1 ± 0.8

24.0 ± 1.4

28.2 ± 1.7

 

36

21.5 ± 3.1

2.7 ± 0.1

20.6 ± 3.0

21.5 ± 1.8

28.1 ± 2.3

 

48

20.4 ± 1.0

2.2 ± 0.3

22.0 ± 2.0

20.8 ± 1.2

28.6 ± 2.7

 

60

19.1 ± 0.1

1.8 ± 0.1

22.4 ± 0.3

20.6 ± 0.1

28.6 ± 0.4

 

72

17.7 ± 0.2

1.6 ± 0.3

21.8 ± 1.3

20.1 ± 2.1

29.0 ± 0.7

Mc-D61

 

24

22.1 ± 0.2

ND

16.5 ± 0.1

17.5 ± 0.1

41.9 ± 0.4

 

36

18.0 ± 0.1

ND

22.2 ± 0.1

12.3 ± 0.0

42.2 ± 0.1

 

48

16.8 ± 0.2

1.4 ± 0.0

22.1 ± 0.2

13.3 ± 0.1

42.6 ± 0.0

 

60

16.2 ± 0.1

1.3 ± 0.3

22.1 ± 0.2

14.4 ± 0.2

42.8 ± 0.2

 

72

14.9 ± 0.1

ND

22.1 ± 0.2

14.2 ± 0.1

43.0 ± 0.6

Mc-D62

 

24

22.5 ± 0.1

4.2 ± 0.0

19.3 ± 0.1

16.6 ± 0.1

36.6 ± 0.2

 

36

22.0 ± 0.5

3.6 ± 0.3

21.5 ± 0.2

15.4 ± 0.1

36.5 ± 0.1

 

48

21.3 ± 0.1

3.2 ± 0.1

22.1 ± 0.2

14.4 ± 0.1

37.0 ± 0.1

 

60

20.2 ± 0.2

3.0 ± 0.1

22.4 ± 0.0

13.6 ± 0.0

37.0 ± 0.1

 

72

18.0 ± 0.1

2.7 ± 0.3

22.8 ± 0.2

13.3 ± 0.1

37.1 ± 0.6

  1. ND not detected
  2. aStrains were cultured in a 2 l fermenter with the 1.5 l Kendrick medium during 72 h. The fatty acid composition were displayed at different point times. The values are means ± standard deviations of two independent experiments