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Table 1 The fatty acid composition in DELTA-12 and DELTA-6 desaturase genes overexpressing strains

From: Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases

Strains Time (h) Fatty acid composition (relative %, w/w)a
16:0 18:0 18:1 18:2 (LA) 18:3 (GLA)
Mc-1552
  24 23.1 ± 0.5 4.0 ± 2.0 23.2 ± 8.7 17.4 ± 5.5 30.0 ± 7.1
  36 23.8 ± 0.5 3.6 ± 0.1 26.2 ± 0.1 14.8 ± 1.6 31.0 ± 1.2
  48 21.1 ± 0.2 2.7 ± 1.3 28.3 ± 2.6 14.5 ± 1.3 31.1 ± 2.6
  60 18.2 ± 0.9 2.2 ± 0.6 24.6 ± 0.2 14.3 ± 1.8 31.2 ± 0.1
  72 17.1 ± 0.6 2.0 ± 0.5 24.0 ± 3.6 14.3 ± 3.2 31.2 ± 0.4
Mc-D12
  24 23.0 ± 8.4 3.0 ± 0.9 12.1 ± 0.8 24.0 ± 1.4 28.2 ± 1.7
  36 21.5 ± 3.1 2.7 ± 0.1 20.6 ± 3.0 21.5 ± 1.8 28.1 ± 2.3
  48 20.4 ± 1.0 2.2 ± 0.3 22.0 ± 2.0 20.8 ± 1.2 28.6 ± 2.7
  60 19.1 ± 0.1 1.8 ± 0.1 22.4 ± 0.3 20.6 ± 0.1 28.6 ± 0.4
  72 17.7 ± 0.2 1.6 ± 0.3 21.8 ± 1.3 20.1 ± 2.1 29.0 ± 0.7
Mc-D61
  24 22.1 ± 0.2 ND 16.5 ± 0.1 17.5 ± 0.1 41.9 ± 0.4
  36 18.0 ± 0.1 ND 22.2 ± 0.1 12.3 ± 0.0 42.2 ± 0.1
  48 16.8 ± 0.2 1.4 ± 0.0 22.1 ± 0.2 13.3 ± 0.1 42.6 ± 0.0
  60 16.2 ± 0.1 1.3 ± 0.3 22.1 ± 0.2 14.4 ± 0.2 42.8 ± 0.2
  72 14.9 ± 0.1 ND 22.1 ± 0.2 14.2 ± 0.1 43.0 ± 0.6
Mc-D62
  24 22.5 ± 0.1 4.2 ± 0.0 19.3 ± 0.1 16.6 ± 0.1 36.6 ± 0.2
  36 22.0 ± 0.5 3.6 ± 0.3 21.5 ± 0.2 15.4 ± 0.1 36.5 ± 0.1
  48 21.3 ± 0.1 3.2 ± 0.1 22.1 ± 0.2 14.4 ± 0.1 37.0 ± 0.1
  60 20.2 ± 0.2 3.0 ± 0.1 22.4 ± 0.0 13.6 ± 0.0 37.0 ± 0.1
  72 18.0 ± 0.1 2.7 ± 0.3 22.8 ± 0.2 13.3 ± 0.1 37.1 ± 0.6
  1. ND not detected
  2. aStrains were cultured in a 2 l fermenter with the 1.5 l Kendrick medium during 72 h. The fatty acid composition were displayed at different point times. The values are means ± standard deviations of two independent experiments