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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Efficient soluble expression of disulfide bonded proteins in the cytoplasm of Escherichia coli in fed-batch fermentations on chemically defined minimal media

Fig. 1

Production of GH1. a, b Representative growth profiles of K-12 strain BW25113 co-expressing coPDI, coErv1p and GH1; a constant temperature growth, b growth at 37 °C to increase cell mass, then shift to 30 °C pre-induction. Error bars represent the standard deviation from 3 samples. F indicates feeding started, I indicates induction. Cells were harvested and protein purified from the last time point shown. c Representative elution profile from IMAC purification of GH1. d Coomassie stained reducing SDS-PAGE analysis of produced proteins: molecular marker, total E. coli lysate (T), soluble protein fraction (S) and IMAC purified GH1. e rpHPLC analysis of purified GH1: comparison of GH1 produced in fed-batch fermentation (red) and in shake flask (black)

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