Fig. 3
From: Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker
Physical map of pKGFP-ld expression vector for multiple copy integration in K. phaffii. leu2-d defective LEU2 allele from S. cerevisiae. P PGK1 and P TEF1 promoters from the K. phaffii PGK1 and TEF1 genes, respectively, P EM7 synthetic E. coli promoter, kan G418/kanamycin resistance gene, EGFP enhanced green fluorescence gene, CYCtt and AOX1tt transcription termination sequences from CYC1 and AOX1 genes, respectively, ori E. coli origin of replication