Fig. 2

Time course analysis of the expression of R0.6C in L. lactis. a Optical density measurements at 600 nm from 0 to 15 h during expression of R0.6C. b Samples collected from 9 to 15 h were analyzed. For Coomassie (20 µl) and WB (10 µl) samples without a reducing agent were loaded on SDS-PAGE gel. Upper panel Coomassie blue-stained 4–12.5% polyacrylamide gel. Middle panel an WB analysis of the same gel shown in the upper panel using anti-His antibody. Lower panel an immune blot analysis of the same gel shown in the upper panel using mAb45.1