Skip to main content
Fig. 1 | Microbial Cell Factories

Fig. 1

From: Construct design, production, and characterization of Plasmodium falciparum 48/45 R0.6C subunit protein produced in Lactococcus lactis as candidate vaccine

Fig. 1

Constructs for expression of R0.6C in L. lactis. a Schematic representation of Pfs48/45 and the R0.6C constructs: 1 C-terminal 6xHis-tag, 2 codon optimized genes (hiligheted with color shades), 3 no tag, 4 N-terminal Spytag, 5 N-termianl SpyCatcher, 6 N-terminal mSA, 7 C-terminal mSA, 8 USP45 signal peptide. The protein yield of each construct was determined by densitometry scanning of the Coomassie stained SDS-PAGE gel shown below. b Upper panel Coomassie blue-stained 4–12.5% polyacrylamide gel for the constructs shown in a. Lower panel an immunoblot analysis of the same gel shown in the upper panel using mAb45.1

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us