The effect of hypoxia on the lipidome of recombinant Pichia pastoris

Table 2 Distribution of the produced Fab 2F5

Culture conditions	D (h⁻¹)	q_Fab (mg_Fab \({\text{g}}_{\text{DCW}}^{ - 1}\) h⁻¹)	Fab expression (mg_Fab \({\text{g}}_{\text{DCW}}^{ - 1}\))				%
Culture conditions	D (h⁻¹)	q_Fab (mg_Fab \({\text{g}}_{\text{DCW}}^{ - 1}\) h⁻¹)	Extracellular fraction	Cytosolic fraction	Membrane fraction	Total	Secretion
Normoxia	0.094 ± 0.005	0.017 ± 0.008	0.254 ± 0.017	0.023 ± 0.001	0.005 ± 0.000	0.282 ± 0.017	90.1 ± 4.7
Hypoxia	0.092 ± 0.005	0.049 ± 0.012*	0.534 ± 0.007*	0.033 ± 0.002*	0.005 ± 0.001	0.572 ± 0.007	93.4 ± 1.6
Fluconazole	0.094 ± 0.006	0.021 ± 0.004	0.223 ± 0.004*	0.024 ± 0.002	0.007 ± 0.001*	0.254 ± 0.004	87.8 ± 2.1
Fluconazole + hypoxia	0.100 ± 0.006	0.033 ± 0.014	0.333 ± 0.015*^†	0.039 ± 0.002*^†	0.012 ± 0.001*^†	0.384 ± 0.015	86.8 ± 5.3

ISSN: 1475-2859