Table 1 Kinetic characterization and enzyme activity of the wild-type and mutant enzymes
Enzyme | Km (μM) | k cat (s−1) | k cat/Km (s−1 M−1) | Maximum enzyme activity (U/mg) | Fold |
|---|---|---|---|---|---|
Sys410 | 14.11 ± 3.22 | 289.12 ± 3.03 | 20.50 | 772.92 ± 3.41 | 1.00 |
M1 | 13.70 ± 2.02 | 293.31 ± 1.27 | 21.41 | 958.43 ± 1.27 | 1.24 |
M2 | 11.61 ± 2.74 | 350.15 ± 6.05 | 30.16 | 1901.31 ± 4.20 | 2.46 |
M3 | 12.77 ± 1.92 | 307.32 ± 4.89 | 24.07 | 1538.14 ± 2.21 | 1.99 |