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Fig. 4 | Microbial Cell Factories

Fig. 4

From: A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis

Fig. 4

SDS-PAGE analysis of the extracellular proteins from different host strains harboring pHY-amyL or pP43SacCNK. a SDS-PAGE analysis of extracellular proteins from tepA and sppA deficient strains harboring pHY-amyL, Lane 1 BL10/pHY-amyL; Lane 2 BL10T/pHY-amyL; Lane 3 BL10S/pHY-amyL. b SDS-PAGE analysis of extracellular proteins from tepA and sppA overexpression strains harboring pHY-amyL, Lane 1 BL10/pHY-amyL; Lane 2 BL10GT/pHY-amyL; Lane 3 BL10GS/pHY-amyL; c SDS-PAGE analysis of extracellular proteins from the tepA and sppA deficient strains harboring pP43SacCNK, Lane 1 BL10/pP43SacCNK; Lane 2 BL10T/pP43SacCNK; Lane 3 BL10S/pP43SacCNK; d SDS-PAGE analysis of extracellular proteins from tepA and sppA overexpression strains harboring pP43SacCNK, Lane 1 BL10/pP43SacCNK; Lane 2 BL10GT/pP43SacCNK; Lane 3 BL10GS/pP43SacCNK. M Protein marker (120, 100, 95, 72, 55, 43, 34, 26, 14 kDa)

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Microbial Cell Factories

ISSN: 1475-2859

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