Fig. 6

Analysis of N-TIMPs on MDA-MB-231 cell behavior in 2D and 3D contact guidance environments. Cells on 2D substrates (A–D) or in 3D environments (E–F) where grouped based on shape and the fraction of cells in each shape were calculated: A 2D spindle, B 2D branched, C 2D bent, D 2D spread, E 3D spindle and F 3D round. Calibration bar is 50 μm. G Fraction of cells that were of different shapes (N _cells  > 79, N _experiments  > 3). Error bars are 95% confidence intervals calculated using Matlab. H Aspect ratio, I migration speed and J directionality in both 2D (black) and 3D (grey) environments (N _cells  > 79, N _experiments  > 3). Error bars are 95% confidence intervals calculated using Matlab. Statistical significance was tested using a t test with p < 0.05 and indicated with bars