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Table 1 Product formation during the growth of C. pasteurianum DSMZ 525 under iron excess (Fe+) and iron limitation (Fe−) conditions. Fermentations were performed in duplicates

From: Metabolic and proteomic analyses of product selectivity and redox regulation in Clostridium pasteurianum grown on glycerol under varied iron availability

 

Biomass

1,3-PDO

BuOH

EtOH

Acetate

Butyrate

Lactate

Formate

Fe+

Fe−

Fe+

Fe−

Fe+

Fe−

Fe+

Fe−

Fe+

Fe−

Fe+

Fe−

Fe+

Fe−

Fe+

Fe−

Final titer (g/L)

5.1 ± 0.09

3.2 ± 0.01

9.4 ± 1.44

16.6 ± 2.26

12.3 ± 0.06

4.4 ± 0.70

1.0 ± 0.12

0.9 ± 0.60

0.8 ± 0.01

1.5 ± 0.02

1.7 ± 0.45

2.8 ± 0.13

0.1 ± 0.00

7.5 ± 2.58

0.5 ± 0.07

1.1 ± 0.11

Production rate (g/L*h) at exponent-ial growth phase

0.39 ± 0.00

0.16 ± 0.03

0.66 ± 0.17

0.74 ± 0.02

0.53 ± 0.11

0.16 ± 0.04

0.06 ± 0.02

0.03 ± 0.003

0.04 ± 0.004

0.07 ± 0.03

0.14 ± 0.02

0.13 ± 0.01

0.007 ± 0.003

0.28 ± 0.02

0.03 ± 0.01

0.04 ± 0.02

 

Y S/X

Y P/X

Y i/X (g/g BM)

9.6 ± 0.3

13.9 ± 1.2

1.16 ± 0.09

4.40 ± 0.52

1.88 ± 0.25

0.97 ± 0.13

0.15 ± 0.06

0.17 ± 0.13

0.10 ± 0.01

0.39 ± 0.09

0.39 ± 0.08

0.80 ± 0.17

0.02 ± 0.00

1.64 ± 0.37

0.08 ± 0.02

0.25 ± 0.05