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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Accelerated triacylglycerol production and altered fatty acid composition in oleaginous microalga Neochloris oleoabundans by overexpression of diacylglycerol acyltransferase 2

Fig. 1

Generation of N. oleoabundans transformants. a Schematic diagram of plasmids pAR-DGAT2 and pB2-DGAT2 used to transform the N. oleoabundans cells. The NeoDGAT2 cDNA (Accession no. GenBank: KJ470774) [11] was expressed by either promoter AR [28], or β2-Tub [29] and contained 3′rbcS2 [41] at 3′end. Hyg3 gene used as selectable marker [40]. The 517-bp PCR amplicon, denoted by a line. b PCR confirmation of NeoDGAT2-expression cassette integration in the transformants. Genomic PCR of transformants AR-DGAT2, B2-DGAT2 and wild type was performed with primers specifically bind to NeoDGAT2 coding sequence. The 517-bp amplicon was detected in the transformants but not in wild type which was used as negative control. Lanes M, 100-bp DNA ladder; C+, plasmid pAR-DGAT2 (used as positive control)

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Microbial Cell Factories

ISSN: 1475-2859

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