Table 1 Expression cassettes and plasmids
From: Production of (S)-2-aminobutyric acid and (S)-2-aminobutanol in Saccharomyces cerevisiae
Feature | Plasmid type | ORF origin | Description | Uniprot number | Reference |
|---|---|---|---|---|---|
pGPD1-ScCHA1-tCYC1 | Entry vector | Saccharomyces cerevisiae | Threonine deaminase | P25379 | This study |
pGPD1-EcILVa-tCYC1 | Entry vector | Escherichia coli | Threonine deaminase | P04968 | [26] |
pGPD1-SlTD-tCYC1 | Entry vector | Solanum lycopersicum | Threonine deaminase | P25306 | [61] |
pGPD1-ScILV1-tCYC1 | Entry vector | Saccharomyces cerevisiae | Threonine deaminase | P00927 | This study |
pGPD1-BsILVa-tCYC1 | Entry vector | Bacillus subtilis | Threonine deaminase | P37946 | [26] |
pPGK1-ScGDH1′-tADH2 | Entry vector | Saccharomyces cerevisiae | Glutamate dehydrogenase (K74V/T177S) | P07262 | This study |
pPGK1-ScGDH3′-tADH2 | Entry vector | Saccharomyces cerevisiae | Glutamate dehydrogenase (K75V/T178S) | P39708 | This study |
pPGK1-EcGDH’-tADH2 | Entry vector | Escherichia coli | Glutamate dehydrogenase (K92V/T195S) | P00370 | [26] |
pPGK1-BcLeuDH-tADH2 | Entry vector | Bacillus cereus | Leucine dehydrogenase | C2RD20 | [8] |
pPGK1-BfLeuDH-tADH2 | Entry vector | Bacillus flexus | Leucine dehydrogenase | A0A0L1MCT3 | This study |
pPGK1-SfValDH-tADH2 | Entry vector | Streptomyces fradiae | Valine dehydrogenase | P40176 | [27] |
pTEF1-NiCAR-tENO2 | Entry vector | Nocardia iowensis | Carboxylic acid reductase | Q6RKB1 | [28] |
pTEF1-NfCAR-tENO2 | Entry vector | Nocardia farcinica | Carboxylic acid reductase | Q5YY80 | This study |
pTEF1-MmCAR-tENO2 | Entry vector | Mycobacterium marinum | Carboxylic acid reductase | B2HN69 | [46] |
pTEF1-MsCAR-tENO2 | Entry vector | Mycobacterium smegmatis | Carboxylic acid reductase | L0IYJ8 | [48] |
pTEF2-BsSFP-tPGI1 | Entry vector | Bacillus subtilis | Phosphopantetheinyl transferase | P39135 | [46] |
pTEF2-MsPPTase-tPGI1 | Entry vector | Mycobacterium smegmatis | Phosphopantetheinyl transferase | L0ITG8 | [48] |
pPDC1-EcALR-tFBA1 | Entry vector | Escherichia coli | Aldehyde reductase | A0A094VUC2 | This study |
pURA3-ScURA3-tURA3 | Entry vector | Saccharomyces cerevisiae | Uracil marker | – | – |
ARS/CEN | Entry vector | Saccharomyces cerevisiae | Origin of replication | – | – |
pPYK1-tTEF1 | 2 micron | Saccharomyces cerevisiae | Vector only | – | – |
pTEF2-tPGI1 | ARS/CEN | Saccharomyces cerevisiae | Vector only | – | – |
pPYK1-ScHOM3-R2-tTEF1 | 2 micron | Saccharomyces cerevisiae | Aspartate kinase (G425D) | P10869 | |
pTEF2-EcGDH’-tPGI1 | ARS/CEN | Escherichia coli | Glutamate dehydrogenase (K92 V/T195S) | P00370 | [26] |