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Table 1 Expression cassettes and plasmids

From: Production of (S)-2-aminobutyric acid and (S)-2-aminobutanol in Saccharomyces cerevisiae

Feature Plasmid type ORF origin Description Uniprot number Reference
pGPD1-ScCHA1-tCYC1 Entry vector Saccharomyces cerevisiae Threonine deaminase P25379 This study
pGPD1-EcILVa-tCYC1 Entry vector Escherichia coli Threonine deaminase P04968 [26]
pGPD1-SlTD-tCYC1 Entry vector Solanum lycopersicum Threonine deaminase P25306 [61]
pGPD1-ScILV1-tCYC1 Entry vector Saccharomyces cerevisiae Threonine deaminase P00927 This study
pGPD1-BsILVa-tCYC1 Entry vector Bacillus subtilis Threonine deaminase P37946 [26]
pPGK1-ScGDH1′-tADH2 Entry vector Saccharomyces cerevisiae Glutamate dehydrogenase (K74V/T177S) P07262 This study
pPGK1-ScGDH3′-tADH2 Entry vector Saccharomyces cerevisiae Glutamate dehydrogenase (K75V/T178S) P39708 This study
pPGK1-EcGDH’-tADH2 Entry vector Escherichia coli Glutamate dehydrogenase (K92V/T195S) P00370 [26]
pPGK1-BcLeuDH-tADH2 Entry vector Bacillus cereus Leucine dehydrogenase C2RD20 [8]
pPGK1-BfLeuDH-tADH2 Entry vector Bacillus flexus Leucine dehydrogenase A0A0L1MCT3 This study
pPGK1-SfValDH-tADH2 Entry vector Streptomyces fradiae Valine dehydrogenase P40176 [27]
pTEF1-NiCAR-tENO2 Entry vector Nocardia iowensis Carboxylic acid reductase Q6RKB1 [28]
pTEF1-NfCAR-tENO2 Entry vector Nocardia farcinica Carboxylic acid reductase Q5YY80 This study
pTEF1-MmCAR-tENO2 Entry vector Mycobacterium marinum Carboxylic acid reductase B2HN69 [46]
pTEF1-MsCAR-tENO2 Entry vector Mycobacterium smegmatis Carboxylic acid reductase L0IYJ8 [48]
pTEF2-BsSFP-tPGI1 Entry vector Bacillus subtilis Phosphopantetheinyl transferase P39135 [46]
pTEF2-MsPPTase-tPGI1 Entry vector Mycobacterium smegmatis Phosphopantetheinyl transferase L0ITG8 [48]
pPDC1-EcALR-tFBA1 Entry vector Escherichia coli Aldehyde reductase A0A094VUC2 This study
pURA3-ScURA3-tURA3 Entry vector Saccharomyces cerevisiae Uracil marker
ARS/CEN Entry vector Saccharomyces cerevisiae Origin of replication
pPYK1-tTEF1 2 micron Saccharomyces cerevisiae Vector only
pTEF2-tPGI1 ARS/CEN Saccharomyces cerevisiae Vector only
pPYK1-ScHOM3-R2-tTEF1 2 micron Saccharomyces cerevisiae Aspartate kinase (G425D) P10869 [31, 32]
pTEF2-EcGDH’-tPGI1 ARS/CEN Escherichia coli Glutamate dehydrogenase (K92 V/T195S) P00370 [26]